Genetic Technology

Worked examples (5)

• 1Restriction endonucleases and sticky ends (5 marks)
• 2Reverse transcriptase and cDNA (5 marks)
• 3PCR — three steps of one cycle (6 marks)
• 4Gel electrophoresis separation principle (5 marks)
• 5Identifying transformed bacteria with marker genes (4 marks)

Model answers (5)

• ✓Model answer — 9700/42 May/Jun 2024 Q8(a) (adapted)
• ✓Model answer — 9700/42 Oct/Nov 2024 Q8(b) (adapted)
• ✓Model answer — 9700/42 May/Jun 2024 Q8(c) (adapted)
• ✓Model answer — 9700/42 Oct/Nov 2024 Q8(c) (adapted)
• ✓Model answer — 9700/42 May/Jun 2024 Q8(d) (adapted)

Worked examples (5)

• 1Producing recombinant human insulin (8 marks)
• 2Advantages of recombinant vs animal-derived insulin (4 marks)
• 3Somatic vs germline gene therapy (4 marks)
• 4CRISPR-Cas9 gene editing (5 marks)
• 5Ethical issues of genetic screening (6 marks)

Model answers (5)

• ✓Model answer — 9700/42 May/Jun 2024 Q9 (adapted)
• ✓Model answer — 9700/22 May/Jun 2024 Q5 (adapted)
• ✓Model answer — 9700/42 Oct/Nov 2024 Q9(b) (adapted)
• ✓Model answer — 9700/42 May/Jun 2024 Q9(b) (adapted)
• ✓Model answer — 9700/42 Oct/Nov 2024 Q9(c) (adapted)

Worked examples (4)

• 1Using Agrobacterium to make a GM plant (6 marks, Paper 4)
• 2Mechanism of Bt resistance to insect pests (5 marks, Paper 4)
• 3Golden Rice — biology and controversy (6 marks, Paper 4)
• 4Risks and benefits of GM crops (6 marks, Paper 4)

Model answers (4)

• ✓Model answer — Adapted from 9700/42 May/Jun 2024 Q7
• ✓Model answer — Adapted from 9700/42 Oct/Nov 2024 Q8
• ✓Model answer — Adapted from 9700/42 May/Jun 2024 Q4
• ✓Model answer — Adapted from 9700/42 Oct/Nov 2024 Q6