Cambridge O Level Biology (5090) – Topical Revision Checklist 2026

Track your progress across cells, biological molecules, plant and animal nutrition, transport, respiration, coordination, reproduction, inheritance and ecology. Aligned to the 2026 Cambridge 5090 syllabus.

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TopicSub-TopicConfidence (1–5)Last ReviewedNext Review
1. Cell biologyCell structure and organelles (animal vs plant cells)
1. Cell biologyLevels of organisation: cells, tissues, organs, systems
1. Cell biologyMovement in and out of cells: diffusion, osmosis, active transport
1. Cell biologyMitosis, meiosis and cell division
1. Cell biologyMicroscope use and cell measurement
2. Biological molecules and enzymesCarbohydrates, lipids and proteins: structure and tests
2. Biological molecules and enzymesEnzyme structure and the lock-and-key model
2. Biological molecules and enzymesEffects of temperature, pH and substrate concentration
2. Biological molecules and enzymesEnzyme applications in industry and food
3. Plant nutrition and transportPhotosynthesis: reactants, products and word equation
3. Plant nutrition and transportLimiting factors in photosynthesis
3. Plant nutrition and transportLeaf structure and gas exchange
3. Plant nutrition and transportTransport in plants: xylem, phloem, transpiration
3. Plant nutrition and transportMineral requirements of plants
4. Animal nutrition and transportBalanced diet, nutrients and deficiency diseases
4. Animal nutrition and transportHuman digestive system and enzyme action
4. Animal nutrition and transportAbsorption and assimilation of food
4. Animal nutrition and transportBlood: components and functions
4. Animal nutrition and transportHeart structure and circulatory system
5. Respiration, gas exchange and excretionAerobic and anaerobic respiration
5. Respiration, gas exchange and excretionHuman gas exchange and breathing
5. Respiration, gas exchange and excretionEffects of smoking on the respiratory system
5. Respiration, gas exchange and excretionKidney structure and excretion
5. Respiration, gas exchange and excretionHomeostasis and temperature regulation
6. Coordination and responseNervous system: neurones and reflex arcs
6. Coordination and responseSense organs: structure of the eye
6. Coordination and responseHormones and endocrine glands
6. Coordination and responseTropisms and plant responses to stimuli
7. Reproduction, inheritance and evolutionAsexual and sexual reproduction in plants and animals
7. Reproduction, inheritance and evolutionHuman reproductive system and menstrual cycle
7. Reproduction, inheritance and evolutionDNA, chromosomes, genes and protein synthesis
7. Reproduction, inheritance and evolutionMonohybrid inheritance and genetic crosses
7. Reproduction, inheritance and evolutionVariation, natural selection and evolution
8. Ecology and human impactEcosystems, food chains and food webs
8. Ecology and human impactNutrient cycles: carbon, water and nitrogen
8. Ecology and human impactPopulations and factors affecting growth
8. Ecology and human impactHuman impact: pollution, deforestation, conservation
8. Ecology and human impactBiotechnology and genetic engineering

Use with our Past Paper Finder for Cambridge O Level Biology 5090 past papers.

Cambridge O Level Biology 5090 revision checklist FAQ

Quick answers about this free revision checklist, how to use it for exam prep, and how it relates to the official syllabus.

What does the 2026 Cambridge O Level Biology 5090 syllabus cover?

This revision checklist mirrors the official Cambridge O Level Biology 5090 syllabus for the 2026 examination series. Every topic and sub-topic on the page is taken from the published syllabus document, so working through the list in order gives you full coverage of what your exam can assess. For the authoritative version, always cross-check with the latest syllabus PDF on your exam board's website before your final revision push.

How many topics are on the Cambridge O Level Biology 5090 exam?

The number of top-level topic groups varies by subject, but you can see the exact count on this page — each major heading in the checklist corresponds to one syllabus topic group, and each row below it is a syllabus-level sub-topic. Use the confidence column (1–5) to flag which sub-topics need more work, and re-score yourself weekly to track real progress instead of guessing.

How long should I spend revising for Cambridge O Level Biology 5090?

Plan back from your exam date, allow at least one week per topic group, and reserve the final 2–3 weeks for full past-paper practice. Use this checklist to plan your weeks: filter by topics you have rated 1–3 and spend your first revision block there. Subjects with heavy practical or extended-writing components (e.g. sciences, English) need more past-paper time in the final block than the topic-by-topic phase.

What's the best order to revise Biology topics?

Revise in roughly the order the syllabus lists the topics — exam boards build later topics on earlier ones, so taking them in syllabus order avoids gaps. Once you have rated every topic, switch to weakest-first: filter the checklist by confidence ≤ 2 and prioritise those topics in your next study block. This is more effective than re-revising topics you already score 4–5 on.

Where can I find Cambridge O Level Biology 5090 past papers and mark schemes?

You can find past papers and mark schemes via Tutopiya's Past Paper Finder and on your exam board's official site. Once you have rated each sub-topic on this checklist, attempt past-paper questions on your weakest topics first — practising under timed conditions is the single best predictor of exam performance, more so than re-reading notes.

How do I download this revision checklist as a PDF or CSV?

Use the Download CSV or Print PDF button at the bottom of the checklist. CSV opens in Excel, Numbers or Google Sheets so you can sort by confidence and re-arrange revision order. The PDF is print-ready for offline use. A free Tutopiya account is required for download — this also unlocks the matching topic resources, notes and worked examples on the Learning Portal.

Is this Biology revision checklist free to use?

Yes, the checklist itself is free — you can view, score and re-score every topic on this page without an account. The CSV / PDF downloads and access to matching Tutopiya Learning Portal resources require a free account. There is no payment required at any point; teachers and parents can also use this checklist freely with their students.

Does this checklist match the latest Cambridge O Level Biology 5090 specification?

Yes. The topics and sub-topics on this page are drawn from the current 2026 Cambridge O Level Biology 5090 specification published by Cambridge. Exam boards occasionally tweak weighting or assessment structure mid-cycle, so do a quick sanity-check against the official syllabus PDF when you start your revision and again 4 weeks before the exam.